Carboxyl ester hydrolase from Penicillium expansum : cloning, characterization and overproduction by Penicillium griseoroseum
نویسندگان
چکیده
منابع مشابه
Morphological and Molecular Differentiation of the Pectinase Producing Fungi Penicillium Expansum and Penicillium Griseoroseum
Two species from the genus Penicillium, Penicillium expansum and P. griseoroseum (Brasilian isolates) were characterized morphologic and molecularlly. Morphological variability was detected among isolates in regard to colony morphology and to conidia coloration. The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. A total of 78 RAPD primers ...
متن کاملMolecular cloning and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene from Penicillium expansum PE-12.
Penicillium expansum produces large amounts of lipase, which is widely used in laundry detergent and leather industry. We isolated the glyceraldehyde-3-phosphate dehydrogenase gene (PeGPD) from P. expansum PE-12 through reverse transcriptase PCR and 5'-3' rapid amplification of cDNA ends (RACE-PCR). The gene is 1266 bp long, including an ORF of 1014 bp, encoding a polypeptide chain of 337 amino...
متن کاملProduction of patulin by Penicillium expansum.
Twenty-seven isolates of Penicillium expansum Lk. ex Thom obtained from Europe, Australia, and North America from seven different fruit hosts all produced patulin in culture. Six isolates were essentially nonpathogenic in apple fruits. In culture, patulin generally accumulated to much higher levels than in apple fruits. At all temperatures permitting fungus growth, patulin was produced. However...
متن کاملPectin lyase overproduction by Penicillium griseoroseum mutants resistant to catabolite repression
Expression of pectinolytic genes is regulated by catabolic repression limiting the production of pectin lyase (PL) if the natural inducer, pectin, is missing from the growth medium. Here, we report the isolation of Penicillium griseoroseum mutants resistant to 2-deoxy-d-glucose (DG) that show resistance to catabolite repression and overproduce PL. Three spontaneous and nine UV-induced mutants w...
متن کاملIdentification and characterization of LysM effectors in Penicillium expansum
P. expansum is regarded as one of the most important postharvest rots of apple fruit and is also of great concern to fruit processing industries. Elucidating the pathogenicity mechanism of this pathogen is of utmost importance for the development of effective and safe management strategies. Although, many studies on modification of the host environment by the pathogen were done, its interaction...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2013
ISSN: 1364-5072
DOI: 10.1111/jam.12215